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Moraes APd, Filho WdSS, Guerra M (2007) Karyotype diversity and the origin of grapefruit. Chromosome Res 15:115–121 In C. sinensis, the expression of CsSWEETs was examined in pollen development. We found that 10 CsSWEETs, including CsSWEET5a, CsSWEET3a, CsSWEET9a, CsSWEET7b, CsSWEET17a, CsSWEET9b, CsSWEET15c, CsSWEET10c, CsSWEET1b, and CsSWEET5c, were highly expressed in one or several pollen stages of C. sinensis ( Figure 4). As previously reported, Chen et al. (2012) identified AtSWEET11 and AtSWEET12 that contributed to phloem loading and pollen nutrition. Gao et al. (2018) found that the OsSWEET11 from O. sativa can greatly affect the starch pollen content. In our study, CsSWEET9b, CsSWEET9a, and CsSWEET10c are the orthologous genes of AtSWEET11 and AtSWEET12, indicating that these highly expressed genes may share similar roles and functions. Stress-Induced Expression Profiles of CsSWEETs in C. sinensis
Related: How to Host a Princess Theme Birthday Party at Home 24 2nd Birthday Party Themes for Girls #1 Two The Moon Li et al. (2017) revealed that Y, P, and D from TMH2, TMH5, and TMH7 were fully conserved residues in pear, respectively ( Li et al., 2017). Among these three residues, Y mutation into A will lead to the loss of transport function in A. thaliana ( Xuan et al., 2013). Proline (P) is a key component of TMHs and allows for dynamic processes during the transport cycle as described by Deber and Therien (2002). In the present study, we suggested that these three residues (Y, P, and D) might contain important functions within the activity of CsSWEETs in C. sinensis. In addition, the mutation of 58 amino acid residues G into D can significantly reduce AtSWEET1 activity in A. thaliana ( Xuan et al., 2013). However, this amino acid is not completely conserved in C. sinensis, including the residue G in CsSWEET12 that had changed to N and in CsSWEET15a that had changed to K, but these proteins also have transport activity ( Figure 2C), which has been verified by RNA-seq. We also noted that the first and the second MtN3/saliva domain included TMH1–TMH3 and TMH5–TMH7, respectively. Microsynteny Analysis Indicates That Large-Scale Duplication Events Contribute to the Expansion for CsSWEET Genes in C. sinensis Guo WW, Wu RC, Cheng YJ, Deng XX (2007) Production and molecular characterization of citrus intergeneric somatic hybrids between red tangerine and citrange. Plant Breed 126:72–76 It’s hard for her to grasp. We bought her all the books – she wasn’t interested in any. All she wanted to do was read the Gruffalo.In our study, transcriptome data from various tissues in the public database NCBI contained three biological replicates. The RNA-seq reads, including seed, flower, stem, root, two and a bud, one and a bud, old leaf, mature leaf, the second leaf, the first leaf, lateral bud, apical bud, early stage lateral bud, CP24 (24 h after pollination; CP, cross-pollinated), CP48, CP72, SP24 (SP, self-pollinated), SP48, and SP72, were obtained from NCBI (PRJNA291116 and PRJNA230752). The Trimmomatic (version 0.33) was used to carried out the quality-based trimming ( Bolger et al., 2014). The HISAT2 was used to map the paired reads to the C. sinensis genome with default parameters ( Kim et al., 2019). The StringTie was used to calculate the fragments per kilobase of exon model per million reads mapped values of differently expressed genes ( Pertea et al., 2016). Results and Discussion The Phylogenetic Analysis Divides CsSWEET Genes Into Four Subfamilies in C. sinensis The Kliq surprises the WWE fans who recorded the MSG "Curtain Call": WWE.com Exclusive, Aug. 28, 2015 NASH: A lot of people are really up in arms about it. Finn is a Superstar. Everyone in WWE is a Superstar, but Finn is a Superstar. Finn is very much like a Shawn Michaels kind of athlete. For him to carry that torch for us, all it does is make our legacy stronger. I wasn’t in a college fraternity, but if I was in Sigma Nu or whatever and your brother underneath you wants to keep the torch alive, I can’t think of a better guy than Finn to do it.
Toolapong P, Komatsu H, Iwamasa M (1996) Triploids and haploid progenies derived from small seeds of‘Banpeiyu’ pummelo, crossed with‘Ruby Red’grapefruit. J Jpn Soc Hortic Sci 65:255–260 The intensity of the yellow or orange color of the sweet potato is directly linked to its beta carotene content ( 41). A medium-sized sweet potato (boiled without the skin) contains 27 grams of carbs. The main components are starches, which make up 53% of the carb content. Froelicher Y, Bassene JB, Jedidi-Neji E, Dambier D, Morillon R, Bernardini G, Costantino G, Ollitrault P (2007) Induced parthenogenesis in mandarin for haploid production: induction procedures and genetic analysis of plantlets. Plant Cell Rep 26:937–944
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Cheng YJ, Guo WW, Yi HL, Pang XM, Deng XX (2003) An effecient protocol for genomic DNA extraction. Plant Mol Biol Rep 21:177a–177g Studies indicate that sweet potatoes’ potent antioxidants may reduce your risk of cancer. Purple potatoes have the highest antioxidant activity ( 14, 51). SUMMARY BÁLOR: We’d be on these six- to eight-hour bus rides on the New Japan tour bus, going from Tokyo to Osaka or to Kyushu, and absolutely going insane on the bus and trying to think of ways to entertain ourselves. We went through this phase of quoting all The Attitude Era stuff. Kobayashi S, Ohgawara T, Saito W, Nakamura Y, Omura M (1997) Production of triploid somatic hybrids in citrus. J Jpn Soc Hortic Sci 66:453–458
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